Fig 1.
The scheme of cloning pGA-BGL and pGA-EGIV plasmids.
Fig 2.
SDS-PAGE of P. verruculosum preparations.
M, molecular markers (in kDa); 1, hBGL1; 2, hBGL2; 3, hBGL3; 4, PvC1; 5, PvC2; 6, hLPMO.
Table 1.
Specific activities (U/mg protein) of enzyme preparations.
Fig 3.
Progress kinetics of Avicel hydrolysis by different P. verruculosum preparations.
Conditions: substrate concentration 100 mg/mL; protein loading 5 mg/g substrate; CDH loading (when applied) 0.1 mg/g substrate; 50°C; pH 5.0.
Fig 4.
Progress kinetics of hydrolysis of pretreated aspen wood by different P. verruculosum preparations.
Conditions: substrate concentration 100 mg/mL; protein loading 5 mg/g substrate; 50°C; pH 5.0.