Fig 1.
Total IFN-γ (Fig 1a) and TNF-α secretion (Fig 1c) after incubation of PBMCs from patients with confirmed Sarcoidosis (S) or healthy volunteers (H) with sarcoidosis (sKv) or control (cKv) Kveim reagent. Each dot represents mean cytokine concentration of one well stimulated in duplicate with antigen for 36 hrs. Fig 1b and 1d represent replicate experiments with the same PBMCs but using replicates of sKv and cKv. Statistical significance for each comparison was determined by Mann-Whitney test.
Table 1.
Fig 2.
Images of colloidal Coomassie Blue stained 1D-SDS-PAGE gels loaded with biological replicates of historical diagnostically in vivo validated Kv (vKv) (Fig 2a), newly created Kv from sarcoidosis spleen (sKv) (Fig 2b) and newly created control Kv from healthy spleen tissue (cKv) (Fig 2c). Each lane was sliced into 10 equal sections. Proteins were subjected to in-gel tryptic digestion and peptides analysed by MS/MS and database searching for protein identification.
Table 2.
Fig 3.
Example of a 2D-DIGE image (pI 6–9) depicting differences in protein abundance between sarcoidosis and healthy control spleen tissue.
Table 3.
Fig 4.
Immunohistochemical staining of spleen tissue shows increased abundance of vimentin in sarcoidosis spleens at 200 x (Fig 4a) and 400 x magnification (Fig 4b) compared to healthy spleen tissue at the same magnifications (Fig 4c and 4d).
Fig 5.
Total IFN-γ (Fig 5a) and TNF-α secretion (Fig 5b) after incubation of PBMCs from patients with sarcoidosis (S), tuberculosis (TB) or from healthy volunteers (H) with pooled recombinant human vimentin. Each dot represents the mean cytokine concentration of one PBMC sample stimulated in duplicate with protein for 36 hrs (after subtraction of the negative control well). Statistical significance between comparisons was determined by Mann-Whitney test.