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Table 1.

The seed coated formulations.

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Table 2.

The effect of inoculation with different rhizobia strains on the number of alfalfa nodules and effective nodules.

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Fig 1.

Plant height (a) and aboveground biomass (b) of alfalfa inoculated with different rhizobia.

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Table 3.

Impact of different concentrations of ammonium molybdate on the growth of rhizobia strains.

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Table 4.

Analysis of variance for the effects of different Mo concentrations, adhesive agents and their interactions on plant height, aboveground biomass, the number of nodules, nodule weight and nitrogenase activity in alfalfa.

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Table 4 Expand

Fig 2.

Plant height and aboveground biomass of alfalfa inoculated with two rhizobia seed coat formulations.

Between the four Mo concentrations under the control treatment, bars that do not share capital letters are significantly different (P < 0.01). The data are presented as means ± SE. *** P < 0.001; NS P > 0.05. Abbreviations are as follows: Adhesive agent (AD), Ammonium molybdate concentration (Mo), Carboxymethyl cellulose (CMC), Sodium alginate (AE), Sodium alginate + skimmed milk (AES).

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Fig 3.

The number, weight and nitrogenase activity of root nodules in alfalfa inoculated with different rhizobia seed coat formulations.

Note: Between the four Mo concentrations under the control treatment, bars that do not share a capital letters are significantly different (P < 0.01). The data are presented as means ± SE. *** P < 0.001;** P < 0.01; NS P > 0.05. Abbreviations are as follows: Adhesive agent (AD), Ammonium molybdate concentration (Mo), Carboxymethyl cellulose (CMC), Sodium alginate (AE), Sodium alginate + skimmed milk (AES).

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Fig 3 Expand