Fig 1.
Mean squared displacment curve for simulated motion models.
Mean squared displacment curves based on simulated trajectories for directed motion (black), normal diffusion (blue), anomalous diffusion (green) and confined diffusion (red).
Table 1.
Confusion matrix based on out-of-bag data.
Table 2.
Sensitivity and specificity for different motion types evaluated by 10-fold cross validation.
Fig 2.
Class-specific mean decrease in accuracy (MDA) for directed motion and normal-, anomalous- and confined-diffusion.
The outer right column shows the MDA over all classes.
Fig 3.
Sensitivity measurements for normal diffusion (A), anomalous diffusion (B), directed motion (C) and confined diffusion (D).
Table 3.
Histogram distances for different resampling rates.
The lowest distance for each method is highlighted in bold.
Fig 4.
Motion class proportions of classified positions of experimental trajectories captured by NanoSight NTA (N = 334), darkfield microscopy (N = 90), confocal laser scanning microscopy (N = 41) and fluorescence microscopy (N = 28).
Fig 5.
Images captured with (A) NTA device and (B) fluorescence microscopy with overlayed trajectories recorded from the whole video sequence.
Red trajectories were classified as normal diffusion, green trajectories were classified as anomalous diffusion, yellow trajectories as confined diffusion. Bar: 5 μm.
Fig 6.
NP trajectories in live V79 fibroblasts as classified by the program.
Several cells are gathered within the field of view (left image). While cell borders are not visible in this focal plane, nuclear envelopes stand out clearly under darkfield illumination (white arrows). A total of 246 particle trajectories was identified as either normal diffusion (red), confined diffusion (yellow), anomalous diffusion (green) or directed motion (magenta). Boxed areas (A-D) show selected cases of directed motion. The time durations of the directed motion were 14.6s (A), 7.4s (B), 10s (C), 6.4s (D1), 10.8s (D2). Note that directed motion ends up with confined or anomalous diffusion (A, B, D) or emanates from a phase of free diffusion (C). Bar: 5 μm.
Fig 7.
Image from confocal laser scanning microscopy overlayed with particle trajectories recorded from the whole video sequence.
Red trajectories were classified as normal diffusion, yellow as confined diffusion, green as anomalous diffusion and magenta as directed motion.