Fig 1.
Schematic representation of L. donovani amastigotes interaction capture & mass spectrometry (MS) analysis.
Live L. donovani amastigotes were UV irradiated to stabilize mRNA-protein interactions. These complexes were isolated by oligo(dT) magnetic beads capture and stringent washings. Bound protein-mRNA complexes were eluted with RNase treatment and identified by MS.
Fig 2.
A and B. Gene Ontology enrichment analysis of L. donovani mRNA interacting proteins.
Fig 3.
Globular domains in L. donovani mRNA interactome.
Number of proteins harboring (A) classical and (B) non-classical RNA binding motifs in L. donovani mRNA interacting proteome. (C) Consolidated number of RNA binding motifs in 79 identified proteins, including 32 unknown RNA binding domains (RBD unknown).
Fig 4.
Detection of L. donovani mRNA interacting proteins in secretome.
(A) Forty three L. donovani mRNA interacting proteins were detected in previously identified L. donovani secretome. (B) Thirty two L. donovani mRNA interacting proteins out of the above 43 proteins were detected in previously identified L. donovani exosome proteome.