Table 1.
Reactions of soybean differentials carrying Rps1a, 1b, 1c, 1d, 1k, 2, 3a, 3b, 3c, 4, 5, 6, 7, and 8 genes to Phytophthora sojae isolates.
Fig 1.
Reactions of soybean differentials carrying Rps4, Rps5, and Rps6 genes and RILs with or without the novel Rps gene to (A) a mixture of P. sojae of R17 and Val12-11 isolates, and (B) the P. sojae P7074 isolate. The presence of a dying or expanded lesion indicates a susceptible response or compatible interaction. Resistance response is expressed as hypersensitive cell death at the inoculation sites and healthy nature of infected plants. AR2, susceptible parent AR2; PI, resistant parent PI399036 containing the novel Rps gene; RIL (R), randomly selected recombinant inbred lines resistant to P. sojae isolates; RIL (S), randomly selected recombinant inbred lines susceptible to P. sojae isolates; Sloan, the susceptible cultivar Sloan with no known Rps genes used as the susceptible control.
Fig 2.
Eleven polymorphic SSR markers linked to Rps12.
SP, susceptible parent AR2; RP, resistant parent PI399036; RB, bulk of 10 resistant homozygous RILs; SB, bulk of 10 susceptible RILs. Satt191, BARCSOYSSR_18_1750; 18_1755, BARCSOYSSR_18_1755; 18_1765, BARCSOYSSR_18_1765; 18_1820, BARCSOYSSR_18_1820; 18_1830, BARCSOYSSR_18_1830; 18_1840, BARCSOYSSR_18_1840; Sat_064, BARCSOYSSR_18_1858; 18_1859, BARCSOYSSR_18_1859; 18_1860, BARCSOYSSR_18_1860; SSRG60684K, SSRG60684K marker; 18_1861, BARCSOYSSR_18_1861.
Fig 3.
Analysis of NBSRps4/6-specific molecular markers linked to a novel Phytophthora resistance gene.
(A) The NBSRps4/6 specific sequence (GenBank accession no. AY258630 [50]) used for developing molecular markers. Primer sequences used for PCR are underlined and marked with half arrows. The PCR primers for amplified targets, NBSRps4/6-1272, NBSRps4/6-869, NBSRps4/6-533 and NBSRps4/6-130, are shown along the primers (S2 Table). (B) The NBSRps4/6 specific molecular markers linked to the novel Rps gene. L, 100 bp DNA Ladder (New England Biolabs, USA); SP, susceptible parent AR2; RP, resistant parent PI399036; RB, bulk of 10 resistant homozygous RILs; SB, bulk of 10 susceptible RILs. NBSRps4/6-533, NBSRps4/6 specific NBSRps4/6-533 marker; NBSRps4/6-130, NBSRps4/6 specific NBSRps4/6-130 marker.
Fig 4.
Genetic and physical map of the Rps12 region.
(A) Molecular genetic map of the Rps loci of the lower arm of Chromosome 18 (S1D Fig). (B) Genetic map of the Rps12 region. SSR markers are shown on the right side of the map and corresponding genetic distances between two adjacent loci are shown on the left side of the map in centi-Morgan (cM). The Rps12 region is shown with a solid line. (C) Physical map of SSR markers on Chromosome 18 according to the soybean reference genome sequence (Glycine max v1.1: http://soybase.org). The corresponding physical distances between two adjacent loci are shown on the left side of the map in mega base pairs (Mb). The Rps12 physical region defined by two molecular markers (BARCSOYSSR_18_1820 and Sat_064) is shown by a solid dark line. (D) Physical location of the Rps12 region on Chromosome 18 (Glycine max v1.1: http://soybase.org). The two long bars indicate two arms of Chromosome 18, the circle indicates the approximate position of the centromeric region, and the marked portion indicates the region containing Rps12.
Fig 5.
Physical map of NBS-LRR like genes present on the Rps12 region.
(A) Genetic map of the Rps12 region (Fig 4B). (B) Physical map of eight NBS-LRR-like genes identified from the Rps12 region of the Williams 82 genome.