Table 1.
Primer sequence use for brain mRNA Analysis.
Fig 1.
Effect of orexin-A injection on body weight evolution.
Data are shown as mean ± SEM, n = 8. A two-factor repeated measures anova was performed to compare both groups and no significant difference was reported.
Fig 2.
Components of caloric intake: total caloric intake (A), meal number (B) meal size (C) and Injestion speed (D). Data are presented as box and whiskers, n = 8. Significant differences are indicated: * P<0.05 (t-test).
Fig 3.
Effect of orexin-A injection on body composition evaluated immediatly after sacrifice.
Data are presented as box and whiskers, n = 8. Significant differences are indicated *P<0.05, ** P<0.01, *** P<0.001 (Student-t test).
Table 2.
Body composition of the control and orexin-A-treated mice (values are in g).
Fig 4.
Components of energy expenditure: energy expenditure.
TEE: total energy expenditure, REE: resting energy expenditure, EE-Act: energy expenditure spent in response to spontaneous motor activity, Activity: spontaneous motor activity, Act-Cost: energy cost of spontaneous activity. Data are presented as box and whiskers, n = 8. A t-test was performed and no significant differences are reported.
Fig 5.
Blood glucose (A) and insulin (B) concentrations during the oral glucose tolerance test in control or orexin-A injected mice. Data are shown as mean ± SD, n = 8. A t-test was performed to compare both groups and no significant difference was reported. Insulin AUC were calculated using the trapezoidal rule (C). Data are presented as box and whiskers, n = 8. A t-test was performed and no significant differences are reported.
Fig 6.
Panel A and B, representative Hematoxylin and Eosin staining (H&E) of pancreas from control (A) and orexin-A-treated (B) mice. Panel C and D, representative OX1R immunostaining of pancreas from control (C) and Orexin-A-treated (D) mice. Arrows highlight the islets. Figures were taken at the same magnification (20x). Scale bar = 50 μm.
Table 3.
Hypothalamus mRNA expression of neuropeptides involved in food intake.