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Table 1.

Sequence information of SSR polymorphism primers (from http://www.cottonmarker.org/cgi-bin/cmd_search_marker.cgi).

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Fig 1.

The images of Gossypium herbaceum (A), G. raimondii (B), Gossypium herbaceum × G. raimondii F1 hybrid (C).

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Fig 2.

Morphological characteristics of G. herbaceum(upper, left), G. raimondii (upper, right) and Gossypium herbaceum × G. raimondii F1 hybrid (lower).

A) Leaf. B) Flower. C) Petals, bracts, calyxs, staminal column and filaments.

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Fig 2 Expand

Fig 3.

Flower characteristics of Gossypiumherbaceum × G. raimondii F1 hybrid.

A-B) Flower. C-D) Staminal column and filaments.

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Fig 3 Expand

Table 2.

Comparison of main characters of G. herbaceum, G. raimondii, and G.herbaceum×G. raimondii.

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Fig 4.

The normal and abnormal meiosis behavior of Gossypium herbaceum × G. raimondii F1 hybrid.

A) Normal dyad. B) Unequal separation of dyad. C) Non-synchronized division of dyad in meiosis II. D) Unequal division with various abnormal micronuclei. E) Abnormal polyads.

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Fig 4 Expand

Fig 5.

Various abnormal pollen grains after meiosis of Gossypium herbaceum × G. raimondii F1 hybrid.

A) Big oval-shaped pollen. B) Small under-developed pollen. C) Deformed pollen. D) Cracked pollen. E) Normal pollen.

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Table 3.

Number of multispores in telophase II of AD hybrid meiosis.

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Table 3 Expand

Fig 6.

Amplification results for three Gossypium herbaceum × G. raimondii F1 hybrids and two parents using 4 representative SSR primer pairs.

A) BNL4053. B) NAU2026. C) NAU1157. D) NAU1164 respectively.1-5: G. herbaceum, hybrid plant 1, hybrid plant 2, hybrid plant 3, and G. raimondii, respectively. The novel bands produced in hybrid plants were indicated by arrows.

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Fig 6 Expand