Table 1.
Baseline characteristics of the patients with septic shock and the controls.
Fig 1.
Immune cell surface antigen expression, lymphocyte count, and TCR diversity in septic shock.
TCR diversity (expressed as a percentage) was calculated as the ratio of the observed number of rearrangements to the theoretical number (A). Lymphocyte count (B), PD-1 expression by CD4+ T cells (C), and HLA-DR expression by CD14+ monocytes (D) measured on day 1, day 3, and day 7 after the diagnosis of septic shock compared with that in healthy volunteers (HV). Data are shown as box plot with medians (lines inside boxes), 25th and 75th quartiles (lines of boxes) and whiskers indicate the range. Any data not included between the whiskers were plotted as an outlier with small circle. **, p<0.01, *p<0.05 vs. healthy volunteers; ††, p<0.01, †, p<0.05 vs. day 1.
Fig 2.
TCR diversity plotted versus PD-1 expression on quadrant charts for days 1, 3, and 7.
Each chart is divided into four zones limited by the normal threshold of TCR diversity (78.3% based on lower quantile data from healthy volunteers) and the normal threshold of PD-1 expression (19.5% based on upper quantile data from healthy volunteers). Patients with CMV reactivation who died are indicated by closed triangles on day 7 and by open triangles on days 1 and 3. Group A: high PD-1 expression with low TCR diversity; group B: high PD-1 expression with normal TCR diversity; group C: normal PD-1 expression with low TCR diversity; group D: normal PD-1 expression with normal TCR diversity. Results are presented as individual values.