Fig 1.
Diagnostic morphological characteristics of Alagoas Curassow.
Pure Alagoas Curassow (A1 and A2), hybrid (B1 and B2), and Razor-billed Curassow (C1 and C2).
Fig 2.
Proportional membership of potential Alagoas Curassows (Pauxi mitu), and Razor-billed Curassows (P. tuberosa) obtained with 14 microsatellite loci.
(A) First stringent analysis performed in NewHybrids. (B) First stringent analysis performed in Structure.
Table 1.
Bayesian analyses with 100 simulated genotypes of each parental and hybrid class, indicating the percentages of simulated individuals correctly assigned as parental or hybrid, independently of hybrid class attribution for both Structure and NewHybrids.
In Structure analyses, potential threshold effect was evaluated using 95, 98 and 99% threshold levels, using K = 2. In NewHybrids, modeling effect was assessed by testing different combinations of Jeffreys (Jef), and Uniform (Un) priors.
Fig 3.
Median Joining network based on 311 bp of the mtDNA control region for 11 Razor-billed Curassows (left circle), and for 72 animals assigned to Alagoas Curassow based on the stringent analyses and Structure analyses using 98% threshold (right circle), with circle sizes reflecting the numbers of individuals.
Green and red colors represent Alagoas and Razor-billed Curassow haplotypes, respectively, indicating Razor-billed maternal ancestry for six individuals assigned to Alagoas Curassow based on morphology and microsatellites. Hatches represent unobserved haplotypes.
Table 2.
Numbers of Razor-billed Curassows (Pauxi tuberosa), and of animals assigned to the Alagoas Curassow (P. mitu) based on morphology and microsatellite analyses, presenting each of two mtDNA control region haplotypes.
Vertical numbers correspond to positions in the sequences deposited in GenBank (accession numbers KU170649 and KU170650) containing diagnostic variations.
Fig 4.
Factorial Correspondence Analysis using 14 microsatellite loci.
General overview of the genetic relationships among Razor-billed Curassows (red); individuals assigned to Alagoas Curassow based on morphology, microsatellites, and mtDNA (green); individuals assigned to Alagoas Curassow based on morphology and microsatellites, but excluded by mtDNA analysis (purple); individuals pointed out as admixted by microsatellites, using Structure 98% threshold (yellow), and individuals assigned as hybrids based on morphology (blue).