Table 1.
Summary of Illumina sequencing of the hooded seal brain transcriptomes.
Table 2.
Summary of the de novo assembly of the hooded seal brain transcriptomes.
Fig 1.
Venn diagram showing the unique and shared DE genes in hypoxia and hypoxia/reoxygenation treated brain slices of the hooded seal.
(A) Upregulated genes, (B) downregulated genes.
Fig 2.
Heat map of gene expression in the visual cortex and brain slices from the hooded seal.
(A) Hierarchical clustering of the regulated genes in the differentially treated brain slices and the visual cortex. (B) Hierarchical clustering of the top 20 up- and top 20 downregulated genes shared between hypoxia/reoxygenation and the normoxia control.
Table 3.
Annotation of hypoxia-regulated genes.
Table 4.
Annotation of hypoxia/reoxygenation-regulated genes.
Fig 3.
Gene ontology analyses of hypoxia and hypoxia/reoxygenation-regulated genes in seal brain slices.
GO analysis in the domain "molecular function" of the significantly up- (A) and down- (B) regulated genes in the hooded seal brain slices after 1 h hypoxia (black) and after 1 h hypoxia followed by 20 min reoxygenation (white). The analyses of the GO terms in the categories "biological process" and "protein class" are given in S3 and S4 Figs.
Fig 4.
Regulation of enzymes of the energy metabolism enzymes in hooded seal cortex in response to hypoxia and reoxygenation.
Upregulated genes are indicated by a green plus (+), downregulated genes are indicated by a red minus (-). All genes show the same patterns of regulation, except Cox5a and Cox5b, which were only regulated by hypoxia, and Atp5i, Sucla2, and Idh3g, which were only found regulated after reoxygenation. Thick arrows represent the reactions that may be favored by the regulations observed here. See S3 Table for abbreviations and details.
Fig 5.
Expression levels of components of the pyruvate dehydrogenase (PDH) complex.
The RPKM values of Pdha1, Pdhx, Dlat and Dld show significant downregulation in the seal brain slices after hypoxia and hypoxia/reoxygenation (N = 3). Error bars represent standard deviations. Statistical significance was indicated by one-way ANOVA associated with Tukey's multiple comparison tests; different letters indicate statistically significant differences.