Table 1.
Secondary metabolites reported from the Wallemia.
Table 2.
Secondary metabolite biosynthetic gene clusters in W. mellicola and W. ichthyophaga identified by the antiSMASH.
Table 3.
The diversity of proteins similar to polyketide synthases and non-ribosomal peptide synthetases in the genomes of W. ichthyophaga and W. mellicola as determined by the psiblast search with homologues from other fungi and confirmed by the phylogenetic analysis.
Fig 1.
Visualization of the predictive clustering tree (PCT) of secondary metabolites in Wallemia constructed by using only the species itself as a descriptive variable.
As target variables the quantity of the 96 secondary metabolites were used. Each of the seven leaves make a prediction of secondary metabolites produced by individual species. In each leaf, the predicted quantities of the secondary metabolites are listed.
Fig 2.
Visualization of the predictive clustering tree (PCT) of secondary metabolites in Wallemia constructed by using the species itself and the water activity as a descriptive variable.
As target variables, the quantities of the 96 secondary metabolites were used. In each leaf, the predicted quantities of the secondary metabolites are listed.
Fig 3.
Visualization of the predictive clustering tree of secondary metabolites in Wallemia constructed by using the solute type and the solute concentrations as descriptive variables.
As target variables the quantities of the secondary metabolites were used: (A) for all species and (B) for W. ichthyophaga, separately. The internal nodes contain tests on individual conditions (e.g., 15% NaCl) and leaves correspond to a specific combination of conditions. In each leaf, the predicted quantities of the secondary metabolites are listed (sorted descending).
Fig 4.
Influence of NaCl on the number of secondary metabolites produced by Wallemia.
Number of secondary metabolites in total (A), ROEDs (D) and YELs (E) on media with different NaCl concentrations (0%, 5%, 10%, 15% and 20%). B-C. Spectrum of ROED (B) and YEL (C) with characteristic peaks at 600 nm and 480 nm, respectively.
Table 4.
Species specific production of biological significant secondary metabolites wallimidione, walleminone, walleminol, UCA 1064-A and UCA 1064-B.
Fig 5.
Production of wallimidione, walleminone and walleminol across the NaCl gradient.