Table 1.
Information about the used strains.
Fig 1.
Workflow of the antimicrobial screening.
From left to right, different plant species were collected from the natural reservation of Ciudad Real, Valencia and Alicante provinces (Spain), plant material was processed and powdered extracts were made. Extracts were used in MABAs at different concentrations to determine the antimicrobial/antifungal activity. To establish the correlation between the antimicrobial activity of the extracts and their composition using HPLC-DAD-ESI-MS/MS analysis was performed.
Table 2.
Plant extracts used in the antimicrobial study.
Table 3.
Selection of extracts.
Fig 2.
Plots for MABA experiments against S. aureus Gram positive bacteria using different concentration of the extracts: 2 mg/mL (A), 1 mg/mL (B) and 0.5 mg/mL (C). Points represent the mean of 12 replicates. Standard error was calculated for each point (data not shown). KM, kanamycin.
Fig 3.
Plots for MABA experiments against E.coli Gram negative bacteria using different concentration of the extracts: 2 mg/mL (A), 1 mg/mL (B) and 0.5 mg/mL (C). Points represent the mean of 12 replicates. Standard error was calculated for each point (data not shown). KM, kanamycin.
Fig 4.
Plots for MABA experiments against C. albicans yeast using different concentration of the extracts: 2 mg/mL (A), 1 mg/mL (B) and 0.5 mg/mL (C). Points represent the mean of 12 replicates. Standard error was calculated for each point (data not shown).
Fig 5.
HPLC chromatogram at 280 nm for HA (A) and CL (B) extracts. Insert in B shows the amplified zone of the profile at 330 for more detailed peak identification. Numbers in the figure correspond to the peaks listed in Table 4 for HA and Table 5 for CL extracts respectively.
Table 4.
MS data for HA extract.
Table 5.
MS data for CL extract.