Fig 1.
(A) UV-visible spectra of AuNPs and pAb-AuNPs, (B) Gel retardation assay of bare AuNPs and pAbs-AuNPs.
Fig 2.
A. Schematic representations showing the LFIA strip assembly. B. Testing of WSSV in LFIA. (a) WSSV negative sample, (b) WSSV neat sample containing purified virus, (c), (d) and (e) correspond to 1:10, 1:20 and 1:40 dilutions of (b) Specificity of LFIA (f) MBV (g) HPV (h) IHHNV. C: Control line, T: Test Line.
Fig 3.
PCR of gill tissue sample from WSSV infected Litopenaeus vannamei.
(A) M: DNA ladder. a: Uninfected gill tissue sample, b: WSSV infected gill sample (Positive control), c to h: Serially double diluted WSSV infected gill sample with concentrations from 100 to 3.125 μg/mL. (B) LFIAs of gill tissue sample from WSSV infected Litopenaeus vannamei. a:Uninfected gill sample b: WSSV infected gill sample (positive control), c to h: Serially double diluted WSSV infected gill tissue sample with a protein content of 100 to 3.125 μg/mL. From these 40 μL was applied in LFIA.
Fig 4.
Standard curve for the real time PCR assay.
(A) The virus was serially diluted (10-fold) from 2.4 x 107 to 2.4 x 102 copies prior to RT-PCR assay. (B) The LOD of LFIA, serially diluted samples were applied to LFIA. Control and Test line were observed visually.
Table 1.
Time course infectivity experiments.
Table 2.
Validation of LFIA.