Table 1.
GenBank accession details for B. mayonii MN1420 and MN14-1539.
Table 2.
Whole genome sequencing and annotation statistics for B. mayonii MN14-1420 and MN14-1539.
Fig 1.
Full plasmid content of B. mayonii.
Visual depiction and annotation of the linear and circular plasmids identified in B. mayonii. Linear plasmid (lp) ideograms were drawn with CViT 1.2.1 and are shown in relation to the size marker in kilobases (kb). Circular plasmid (cp) ideograms were drawn with Circos 0.69, circular plasmids are not drawn to scale. Annotation data was derived from the NCBI Prokaryotic Genome Annotation Pipeline. Proteins annotated as hypothetical (blue), lipoprotein (red), known function (pink), or plasmid partitioning (green) are indicated. Also shown are pseudogenes (black), the silent vls cassettes (light blue) and GC skew (orange lines), lp5 was only present in MN14-1420. lp28-10 is ~4 kbp shorter in MN14-1539.
Table 3.
Nucleotide differences detected between B. mayonii strains MN14-1420 and MN14-1539*.
Fig 2.
Plasmid based protein composition of B. mayonii in comparison to 4 other Bbsl genospecies and 1 RF Borrelia species.
The mean amino acid identity is indicated for proteins present on each of the 14 B. mayonii plasmids. Comparisons are to protein databases constructed for B. burgdorferi (B.b.s.s.; green), B. garinii (B.g.; blue), B. afzelii (B. a.; red), B. bissettii (B.b.; yellow), and B. miyamotoi (B.m.; grey).
Fig 3.
B. mayonii proteins with greatest sequence diversity as compared to 4 other Bbsl genospecies and 1 RF Borrelia species.
Bar graph depicting B. mayonii proteins greater than 100 amino acids with less than 60% amino acid identity to other Bbsl proteins. Amino acid identity is shown with respect to homologs present in B. burgdorferi (B.b.s.s.; green), B. garinii (B.g.; blue), B. afzelii (B. a.; red), B. bissettii (B.b.; yellow) and B. miyamotoi (B.m.; grey).
Fig 4.
Phylogenetic analysis of B. mayonii based on a ~6.5 kbp region of cp26.
The ~6.5 kbp region of cp26 encompasses open reading frames from BB_B14 through BB_B18 of B. burgdorferi B31. DNA sequences from 8 other Bbsl genospecies (22 strains) were retrieved from GenBank. Bootstrap values greater than 50% are shown. The scale bar corresponds to 0.02 substitutions per nucleotide position.
Fig 5.
Comparison of B. burgdorferi B31 and B. mayonii lp54.
Heat map of nucleotide identity between lp54 plasmids of B. burgdorferi B31 (top) and B. mayonii MN14-1420 (bottom). Nucleotide identity along the entire lp54 plasmids is displayed as colored blocks (Yellow = highest, Blue = lowest). Orientation of arrows indicates gene orientation on the forward or reverse DNA strand. The variable PF54 gene array is at the 3’ end of the plasmid. Specific gene loci discussed in text are indicated.
Fig 6.
vls containing linear plasmid lp28-10 from B. mayonii MN14-1420 and MN14-1539.
A) Heat map displaying nucleotide identity (blue—lowest; yellow—highest) between the active vlsE nucleotide sequence (magenta arrow) and silent vls cassettes within the two B. mayonii strains. Position and copy number of the silent cassette array is indicated by the blue to yellow ribbons. Red bars indicate silent vls loci present in MN14-1420 and missing in MN14-1539. Genes flanking the vls locus are indicated by black arrows. Orientation of arrows indicates gene orientation on the forward or reverse DNA strand. B) Multi-alignment of the 26 amino acid C6 peptide within VlsE from B. mayonii, B. burgdorferi B31 and B. garinii IP90. Identical (*), conserved (:) and differentially charged (.) amino acid residues are indicated.
Table 4.
Putative B. burgdorferi plasmid-borne proteins absent from the B. mayonii genome.