Fig 1.
Effects of three antifouling biocides, TBT, diuron, and irgarol on MDA content in the gill of Crassostrea gigas.
Data are presented as the mean ± standard deviation (S.D.). Significant difference compared with control value is indicated by an asterisk (*) on the data bar (p < 0.05).
Fig 2.
Effects of three antifouling biocides, TBT, diuron, and irgarol on antioxidant defense system in the gill of Crassostrea gigas.
A) Results of TBT-exposed oysters. B) Results of diuron-exposed oysters. C) Results of irgarol-exposed oysters. The remaining activities were recorded as percentages relative to the control. Data are presented as the mean ± standard deviation (S.D.). Significant difference compared with control value is indicated by an asterisk (*) on the data bar (p < 0.05).
Fig 3.
Transcriptional profiles of Crassostrea gigas antioxidant defense system in response to different concentrations of TBT, diuron, and irgarol.
Heat map analysis for effects of TBT, diuron, and irgarol on transcriptional expressions of Hsp superfamily.
Fig 4.
Effects of three antifouling biocides, TBT, diuron, and irgarol on Na+/K+-ATPase in the gill of Crassostrea gigas.
A) Effects of TBT, diuron, and irgarol on enzymatic activity of Na+/K+-ATPase. B) Effects of TBT, diuron, and irgarol on mRNA expression of Na+/K+-ATPase α subunit. Data are presented as the mean ± standard deviation (S.D.). Significant difference compared with control value is indicated by an asterisk (*) on the data bar (p < 0.05).
Fig 5.
Effects of three antifouling biocides, TBT, diuron, and irgarol on acetylcholinesterase (AChE) in the gill of Crassostrea gigas.
A) Effects of TBT, diuron, and irgarol on enzymatic activity of AChE. B) Effects of TBT, diuron, and irgarol on mRNA expression of AChE gene. Data are presented as the mean ± standard deviation (S.D.). Significant difference compared with control value is indicated by an asterisk (*) on the data bar (p < 0.05).
Fig 6.
Transcriptional profiles of Crassostrea gigas heat shock protein (Hsp) superfamily in response to different concentrations of TBT, diuron, and irgarol.
A) Heat map analysis for effects of TBT, diuron, and irgarol on transcriptional expressions of Hsp superfamily. B) Expression profiles are represented by a heat map with hierarchical clustering analysis.