Table 1.
Clinical parameters.
Fig 1.
Plasma levels of adiponectin (A), leptin (B) and CRP (C) and dispersion graph of adiponectin versus leptin levels (D) are shown. The groups evaluated were normal-weight (NW; n = 9) and childhood obesity (CO; n = 11). Results in panels A, B and C are presented as mean ± SD. Significant differences (p<0.05) in the charts are identified by p value and connecting lines.
Fig 2.
Frequency of monocytes subsets.
(A) Representative gates of flow cytometry for classification of subsets. (B) Frequency of each monocyte subsets in normal-weight (NW; n = 9) and childhood obesity (CO; n = 11). Graphs shown median with interquartile range. Significant differences (p<0.05) in the charts are identified by asterisks and connecting lines.
Fig 3.
Evaluation of expression of recognition and activation molecules in monocytes and their subsets.
(A) Expression of recognition molecules was determined by mean fluorescence intensity (MFI). (B) Expression of activation molecules was determined by mean fluorescence intensity (MFI). The groups evaluated were normal-weight (NW; n = 9) and childhood obesity (CO; n = 11). Significant differences (p<0.05) between monocytes subsets, intragroup, are identified by asterisks and connecting lines. Letters represent statistical differences (p<0.05) between the same subset for comparison intergroup. Graphs shown median with interquartile range.
Fig 4.
Evaluation of cytokine expression in monocytes and their subsets.
(A) Perceptual of classical monocytes expressing each cytokine. (B) Perceptual of intermediate monocytes expressing each cytokine. (C) Perceptual of non-classical monocytes expressing each cytokine. Graphs show median with interquartile range. Significant differences (p<0.05) are identified by asterisks. (D) Radar graph represent perceptual of each monocytes subsets expressing cytokine. The groups evaluated were normal-weight (NW; n = 9; dispersion points) and childhood obesity (CO; n = 11; bars).
Fig 5.
Heatmap for correlations between inflammatory and anti-inflammatory molecules.
Representative heatmap of correlation between recognition/activation molecules and clinical parameters for classical (A), intermediate (B) and non-classical monocytes (C). Representative heatmap of correlation between cytokines and clinical parameters for classical (D), intermediate (E) and non-classical monocytes (F). The groups evaluated were normal-weight (NW; n = 9) and childhood obesity (CO; n = 11).
Fig 6.
Correlation networks in monocyte subsets.
Correlations network in normal-weight group (NW; n = 9) for classical (A), intermediate (B) and non-classical monocytes (C). Correlations network in childhood obesity group (CO; n = 11) for classical (D), intermediate (E) and non-classical monocytes (F). All correlations shown in networks are strong, presents r-squared higher than 0.63, and statistical significance defined by p<0.05. The continuous lines represent positive correlation and the traced lines represent the negative correlation.