Fig 1.
(A) anion-exchange chromatography elution profiles of crude UP on a column of DEAE-cellulose; (B) HPGPC chromatogram of UP2.
Fig 2.
(A) FT-IR spectrum of UP2; (B) FT-IR spectrum of the methylated polysaccharide.
Fig 3.
(A) GC chromatogram showing the monosaccharide composition of UP2; (B) GC chromatogram showing the monosaccharide composition of UP2-0.5M.
Table 1.
GC-MS date for methylation analysis of UP2.
Fig 4.
(A) 1H NMR spectrum of UP2 in D2O; (B) 13C NMR spectrum of UP2 in D2O; (C) HSQC NMR spectrum of UP2 in D2O; (D) HMBC NMR spectrum of UP2 in D2O.
Table 2.
Chemical shifts of resonances in the 1H NMR and 13C NMR spectra of UP2.
Fig 5.
Predicted structure of the repeating unit of UP2.
Fig 6.
Effect of UP2 on the cell proliferation (A), phagocytosis activity (B), and NO production (C) of RAW264.7 macrophages. All experiments were repeated at least three times. The data values are expressed as mean ± SD (n ≥ 3). Significant difference: *P < 0.05, and **P < 0.01 for difference from the control without treatment.