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Fig 1.

Experimental protocol.

First, the subjects had a 30-s pre-rest. Then, the subjects were given an olfactory stimulus for 10 s, followed by a 30-s post-rest period and a 30-s period to complete a questionnaire on odor intensity and subjective assessments of hedonic and irritating odors. The 130-s cycles for each odorant were repeated six times consecutively.

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Table 1.

Demographic characteristics of the study population.

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Fig 2.

Detection thresholds (A) and recognition thresholds (B) by patients with multiple chemical sensitivity (MCS) (n = 10) and controls (n = 6) in olfactory ability testing.

Y-axes present the mean values of five odorants.

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Fig 3.

Rating of odor intensity (A) as well as hedonic (B) and irritating odors (C) by patients with multiple chemical sensitivity (MCS) (n = 10) and controls (n = 6) after olfactory stimulation.

Abbreviations: D1, γ-undecalactone and concentration level 1; D4, γ-undecalactone and concentration level 4; E1, skatole and concentration level 1; E4, skatole and concentration level 4; and NO, non-odorant. Numbers in parentheses in column 1 indicate the order of the six repetitions (1–6). Statistically significant differences between groups are indicated: *p < 0.05.

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Fig 4.

Time-course of average z-scores of all channels for oxygenated hemoglobin (oxyHb) levels in patients with multiple chemical sensitivity (MCS) (n = 10) and controls (n = 6) during pre-rest (baseline, 10–30 s), stimulation (30–40 s), and post-rest (recovery, 40–70 s).

Y- and X-axes present the z-scored oxyHb values and times, respectively. Signals reflecting the oxyHb concentration changes in arbitrary units were calculated (millimolar–millimeter). The signal data were adjusted using a fast Fourier transform filter smoothing technique (OriginPro 2016 software of OriginLab Corporation). The cutoff frequency was determined at 35 points. The MCS group is indicated as a black line, and the control group is indicated as a gray line. Abbreviations: D1, γ-undecalactone, and concentration level 1; D4, γ-undecalactone, and concentration level 4; E1, skatole, and concentration level 1; E4, skatole, and concentration level 4; and NO, non-odorant. Numbers in parentheses in column 1 indicate the order of the six repetitions (1–6).

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Fig 5.

Topographical maps of average z-scores for oxygenated hemoglobin (oxyHb) levels between patients with multiple chemical sensitivity (MCS) (n = 10) and controls (n = 6).

Abbreviations: D1, γ-undecalactone, and concentration level 1; D4, γ-undecalactone, and concentration level 4; E1, skatole, and concentration level 1; E4, skatole, and concentration level 4; and NO, non-odorant. Numbers in parentheses in column 1 indicate the order of the six repetitions (1–6).

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Table 2.

The t-test results for all channels (1–52) comparing z-scores for oxygenated hemoglobin levels between patients with multiple chemical sensitivity (MCS) and controls.

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Table 2 Expand