Table 1.
Reagents employed.
Table 2.
Instruments employed.
Fig 1.
(A) Surgical preparation time (from heparin administration to flushing; R = 0.291, p = 0.397). (B) Procurement time (from flushing to harvest; R = 0.660, p = 0.0003); (C) Connection time (from harvest to lung connection to the circuit; R = 0.299, p = 0.370). Nonlinear regression analysis.
Fig 2.
Schematic overview of EVLP protocol. Abbreviations: VT, tidal volume; RR, respiratory rate; PEEP, positive end expiratory pressure.
Table 3.
Changes over time in perfusion fluid parameters.
Fig 3.
Lung parameter changes during EVLP.
(A) Pulmonary artery pressure (PAP). (B) Total pulmonary vascular resistance (TPVR). (C) Peak inspiratory pressure (Ppeak). (D) Dynamic compliance (Cdyn). Pressure recruitment maneuvers (RMs) were performed at 40 and 175 min. Results are expressed as the mean ± SEM (N = 10). One-way repeated measures ANOVA; p value was <0.001 for each parameter considered. Tukey’s multiple comparison test.
Table 4.
General protocol features and perfusion fluid characteristics of recently reported EVLP rat models.
Table 5.
Comparison of reperfusion phase settings in recently reported rat EVLP protocols.
Table 6.
Comparison of reconditioning phase settings in recently reported rat EVLP protocols.