Table 1.
Information of the primers and corresponding candidate reference genes.
Fig 1.
Range of Cq values of the nine candidate reference genes across all samples of A. paragallinarum serovars A, B, and C. Boxes and whiskers represent interquartile ranges and confidence intervals. Bars inside boxes indicate median values. Hollow circles show outliers (5th/95th percentile) respectively.
Table 2.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar A analyzed by geNorm.
Table 3.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar B analyzed by geNorm.
Table 4.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar C analyzed by geNorm.
Table 5.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar A analyzed by NormFinder.
Table 6.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar B analyzed by NormFinder.
Table 7.
Gene expression stability rankings for different growth phase in A. paragallinarum serovar C analyzed by NormFinder.
Table 8.
Gene expression stability assessed by the comparative ΔCT method.
Table 9.
Candidate reference genes ranked by different methods in serovar A.
Table 10.
Candidate reference genes ranked by different methods in serovar B.
Table 11.
Candidate reference genes ranked by different methods in serovar C.
Fig 2.
50S ribosomal protein L33 expression analysis at different time points in stationary phase in cultures of serovar B of A. paragallinarum.
Different colored columns show the relative quantification of 50S ribosomal protein L33 when normalized against reference genes at three sampling time points. RecN and 16S rRNA were the two highest ranked genes, gyrA and atpD were the two highly ranked genes for cultures of serovar B of A. paragallinarum in stationary phase and sodA was a poorly ranked gene.