Fig 1.
GTG-banded karyotypes of studied species.
a–L. mandarinus, b–L. brandtii, c–L. gregalis. Black dots mark the position of centromeres. Vertical black bars mark the localization of M. agrestis (MAG) chromosome painting probes, while vertical grey bars mark the localization of D. torquatus (DTO) painting probes. Numbers along the vertical lines correspond to chromosome numbers of M. agrestis and D. torquatus. Black triangles indicate sites of localization of rDNA clusters; grey triangles indicate localization of the largest interstitial telomeric blocks.
Fig 2.
a–L. mandarinus, b–L. brandtii, с –L. gregalis. Scale bar is 10 μm.
Fig 3.
Examples of fluorescence in situ hybridization.
a–MAGX (green) and MAG13-14 (red) onto L. mandarinus chromosomes, b–DTO10-12 (green) and DTO2 (red) onto L. mandarinus chromosomes, c–DTO13 (green) and DTO9 (red) onto L. gregalis chromosomes, d–DTO2 (green) and DTO19 (red) onto L. brandtii chromosomes. Examples of fluorescence in situ hybridization of the 18S/28S-rDNA probe (green) and telomeric DNA probe (red): e–L. mandarinus (white arrows indicate localization of the largest interstitial telomeric blocks), f–L. brandtii, g–L. gregalis. Scale bar is 10 μm.
Table 1.
Distribution of shared syntenic segment associations.
Fig 4.
Karyotype evolution pathways in three Lasiopodomys species.
Tree topology is based on the molecular phylogeny of Arvicolinae species presented by [3]. AAK–ancestral Arvicolinae karyotype, AMiK–ancestral karyotype of the tribe Arvicolini, LAK–ancestral karyotype of the genus Lasiopodomys, sLAK–ancestral karyotype of the subgenus Lasiopodomys. Chromosome numbers are indicated in AAK, LAK, and sLAK segments. *–see Discussion.