Table 1.
Percentage similarity between the amino acid sequence of voltage-gated Na+ channel (Scn) type IV α-subunit isoforms (Scn4aa and Scn4ab) of Electrophorus electricus as compared with Scn4aa and Scn4ab of other teleosts obtained from GenBank.
Sequences are arranged in descending order of similarity. Similarity indices were obtained using the BioEdit software.
Table 2.
Percentage similarity between the amino acid sequence of voltage-gated Na+ channel (Scn) β-subunit isoforms (Scn1b, Scn2b and Scn4b) of Electrophorus electricus in comparison with Scnb/SCNB of other animal species obtained from GenBank.
Sequences are arranged in descending order of similarity. Similarity indices were obtained using the BioEdit software.
Fig 1.
mRNA expression levels of the voltage-gated Na+ channel type IV α-subunit a isoform (scn4aa) in the electric organs (EOs) and the skeletal muscle (SM) of Electrophorus electricus.
Absolute quantification (×103 copies of transcript per ng of cDNA) of scn4aa transcripts in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus kept in fresh water. Results represent means ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).
Fig 2.
mRNA expression levels of the voltage-gated Na+ channel type IV α-subunit b isoform (scn4ab) in the electric organs (EOs) and the skeletal muscle (SM) Electrophorus electricus.
Absolute quantification (× 103 copies of transcript per ng of cDNA) of scn4ab transcripts in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus kept in fresh water. Results represent means ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).
Fig 3.
mRNA expression levels of the voltage-gated Na+ channel β-subunit 1 isoform (scn1b) in the electric organs (EOs) and the skeletal muscle (SM) Electrophorus electricus.
Absolute quantification (copies of transcript per ng of cDNA) of scn1b transcripts in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus kept in fresh water. Results represent means ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).
Fig 4.
mRNA expression levels of voltage-gated Na+ channel β-subunit 2 isoform (scn2b) in the electric organs (EOs) and the skeletal muscle (SM) Electrophorus electricus.
Absolute quantification (copies of transcript per ng of cDNA) of scn2b transcripts in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus kept in fresh water. Results represent means ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).
Fig 5.
mRNA expression levels of voltage-gated Na+ channel β-subunit 4 isoform (scn4b) in the electric organs (EOs) and the skeletal muscle (SM) Electrophorus electricus.
Absolute quantification (× 103 copies of transcript per ng of cDNA) of mRNA expression of scn4b in the main EO, the Hunter’s EO, the Sach’s EO and the SM of the (a) adult or (b) juvenile E. electricus kept in fresh water. Results represent means ± S.E.M. (N = 4). Means not sharing the same letter are significantly different (P<0.05).
Fig 6.
Protein abundances of voltage-gated Na+ channel type IV α-subunit b isoform (Scn4ab) in the electric organs (EOs) and the skeletal muscle (SM) of Electrophorus electricus.
Representative immunoblots and protein abundances of Scn4ab in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus. Protein abundance is expressed as arbitrary densitometric units per 20 μg protein. Results represent mean ± S.E.M (N = 3). Means not sharing the same letter are significantly different (P < 0.05).
Fig 7.
Protein abundances of voltage-gated Na+ channel β-subunit 4 isoform (Scn4b) in the electric organs (EOs) and the skeletal muscle (SM) of Electrophorus electricus.
Representative immunoblots and protein abundances of Scn4b in the main EO, the Hunter’s EO, the Sach’s EO and the SM of (a) adult or (b) juvenile E. electricus. Protein abundance is expressed as arbitrary densitometric units per 20 μg protein. Results represent mean ± S.E.M (N = 3). Means not sharing the same letter are significantly different (P < 0.05).