Fig 1.
The watersheds are indicated by overlaid colors on the green map background. Sampling sites not associated with watersheds along the waterways are indicated with X1 and X2 on the map. Sampling locations were grouped into watersheds to allow statistical analysis as described [26]. Watershed areas are shaded labeled. Salinas River watershed is indicated with the blue line along the Salinas River; Tembladero is the yellow box; Gabilan is the pink box; and Alisal is the orange box. The Carr Lake watershed is between the Tembladero and Alisal watersheds within the city of Salinas. The base layer of the map is from the DEMIS Mapserver. (http://www.demis.nl/home/pages/wms/demiswms.htm), which are public domain. DEMIS World Map Server generates maps using public domain data with no usage restrictions.
Table 1.
Summary of Predicted InlA lengths among 112 isolates.
Fig 2.
Lengths of predicted InlA proteins with functional regions labeled.
The N and C termini are labeled. The final number of the right of each construct is the predicted lengths of the proteins. The InlA protein is divided into 5 regions with the first 29 amino acids (gray region) encoding a Signal Sequence. Amino acids 30–357 (red) encode a leucine rich repeat needed for attachment to the E-cadherin receptor. Amino acids 358–462 (yellow) encode an intergenic repeat. Amino acids 463–650 (green) encode the B repeats section, and amino acids 651–800 encode the Membrane Anchor region. An “L” on the Membrane Spanning region of the top two constructs indicate where the LPXTG motif is located. The region that is deleted in the 797-amino acid protein in relation to the full-length protein is indicated by an asterisk (*).
Table 2.
Sample and isolate information for aberrant InlA types.
Fig 3.
Dendrogram of intact alleles of inlA, and associated information.
Alleles labeled with “1” and letters indicate sequences that were found in more than one isolate. Alleles labeled with “RM” and a number are unique alleles. Also listed are the Lineage information and MLVA types in which the alleles are found, watershed information, sample dates, and the number of isolates of each allele. Watershed information: A, Alisal; C, Carr Lake, G, Gabilan; S, Salinas River; T, Tembladero; X1 –X3, sites not associated with watersheds.
Fig 4.
Alignment of Intact Alleles Showing SNP changes.
The entire 2403 bp length of inlA is shown with the 5 regions as described in the caption to Fig 2. Bases 1–87 (gray) is the signal sequence, bases 88–1071 (red) is the leucine rich repeat region, bases 1072–1386 (yellow) is the intergenic region, bases 1387–1950 (green) is the B repeat region, and bases 1388–2403 (purple) is the membrane anchor region. The boxed alleles were found only in Lineage II strains, while the unboxed alleles were found only in Lineage I strains.