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Fig 1.

The flowchart of the development and evaluation of the diagnostic classification protocol.

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Table 1.

Demographic and clinical data of participants.

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Fig 2.

Feature selection.

Left (VBM): Grey matter. Affected cortical regions at p<0.15 FWE are displayed in red, and the corresponding anatomical label, the precentral gyrus is shown in green. Middle and Right (DTI): White matter. Factional anisotropy (FA—Top) and Radial diffusivity (RD–Bottom) group comparison results are shown in red at p<0.01 FEW-TFCE. The corresponding anatomical labels colour coded as follows: dark green—lateral fibres of the corona radiata, blue—body of the corpus callosum, turquoise—genu of the corpus callosum, purple–splenium of the corpus callosum, bright yellow–inferior corona radiata, green—internal capsule, beige—mesencephalic cruri of the cerebral peduncles, grey–inferior corticospinal tracts in the pons.

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Fig 3.

Classification accuracy in the training sample.

The probability of individual participant’s MRI data demonstrating ALS-specific change based on the classification algorithm. Patients with ALS are represented by filled circles, healthy controls by empty circles. Misclassified participants are displayed in red; correctly classified participants in green.

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Fig 4.

Classification accuracy in the validation sample.

The probability of individual participant’s MRI data demonstrating ALS-specific change based on the classification algorithm. Patients with ALS are represented by filled circles, healthy controls by empty circles. Misclassified participants are displayed in red; correctly classified participants in green.

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Fig 5.

Classification accuracy in the follow-up validation sample.

The probability of individual participant’s MRI data demonstrating ALS-specific change based on the classification algorithm. Patients with ALS are represented by filled circles, healthy controls by empty circles. Misclassified participants are displayed in red; correctly classified participants in green.

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Table 2.

Classification results using the 50% probability threshold.

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Table 2 Expand

Table 3.

Comparison of correctly classified and misclassified ALS patients and controls in the training sample.

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Table 4.

Comparison of correctly classified and misclassified ALS patients and controls in the validation sample.

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Table 5.

Comparison of correctly classified and misclassified ALS patients and controls in the follow-up validation sample.

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Table 5 Expand