Fig 2.
Expression of Fgfr2 in E10.5 in control and Fgfr2ST−/− embryos.
A,C. Lower power (A) and higher power (C) images show that control embryos have a wide band of Fgfr2 signal (between arrowheads) encompassing the Wolffian duct and surrounding stroma. B,D. Lower power (B) and higher power (D) images show that Fgfr2ST−/− embryos have a linear band of Fgfr2 expression in the Wolffian duct epithelium (arrows) and not in the surrounding stroma. E. Quantitative real-time PCR of FAC-sorted E10.5 Cag-Tbx18creTg/+ (Control) and Cag-Fgfr2ST−/−(Fgfr2St−/−) urogenital ridges confirms a dramatic decrease in Fgfr2 mRNA expression in mutant Tbx18cre expressing cells. Scale bars = 100 μm. ***p<0.001 vs. control embryos.