Fig 1.
HPLC chromatogram at λ = 263 nm of a derivatized honey sample displaying peaks of interest.
Dihydroxyacetone (6.56 min), anisole internal standard (7.40 min), 5-hydroxymethylfurfural (8.32 min) and methylglyoxal (12.02 min).
Fig 2.
Quantitation graphs used to calculate mass (μg) of analyte in each calibration standard.
Mass plotted against ratio of analyte peak area to anisole internal standard peak area at λ = 263 nm. (a) Methylglyoxal-bisPFBHA derivative. (b) AccuStandard grade methylglyoxal. (c) Dihydroxyacetone. (d) 5-Hydroxymethylfurfural.
Table 1.
Methylglyoxal, dihydroxyacetone and 5-hydroxymethylfurufal concentrations for Leptospermum honeys.
Table 2.
Comparison between theoretical concentration of commercial methylglyoxal solutions and the results of quantitation method using the anisole technique.