Fig 1.
High-speed backcrossing through the female germline.
(A) Approximate duration of approaches to obtain congenic strains, based on 5 generations for marker-assisted strategies (high-speed, speed) and 9 generations for traditional backcrossing (normal). Assumed durations: gestation, 20 days; weaning/genotyping, 21 days; breeding, 32 days for speed and normal (assuming maturity of males at 7.5 weeks), and 5 days for high-speed (superovulation at pre-pubertal age). (B) Diagram illustrating the experimental approach for high-speed backcrossing through the female germline. Star, mutation of interest.
Table 1.
Backcrossing Outcomes.