Table 1.
Simulated respiratory secretion components and ranges.
Table 2.
Per swab quantities of respiratory sample components in NPS samples from children and adults.
Table 3.
Mean effect and p-values for the fractional factorial testing for the RATs.
Table 4.
Mean effect and p-values for the fractional factorial testing for the NAATs and virus culture.
Fig 1.
The results of testing each component’s effect on the Liat assay.
H1N1pdm was diluted in each of the matrices containing each of the components and tested in triplicate in the Liat assay. The experiment was performed twice with the repeats shown in black and red. Each matrix was compared to PBS using the t-test. Bars indicate the mean of the three replicates for each repeat. Asterisks indicate a significant difference from PBS alone.
Fig 2.
Results of testing virus spiked in PBS, SRS, and NCS with six assays.
Ct and antigen assay signal results were normalized as a percentage of the PBS result value. Data for high and low concentrations of virus are shown.
Table 5.
P-values from statistical analysis of matrix comparison data.
Table 6.
Serial dilutions of viruses in SRS in the Simplexa, Liat, and Alere assays.
Table 7.
Results of testing 10-fold serial dilutions of infected cells with each assay.
Table 8.
Comparing the effect of SRS versus PBS on the detection of infected cells or cell free virus (analytes).
(SRS Value/ PBS value).
Fig 3.
(A) Dot blot for the detection of IgG antibodies specific to the H1N1pdm NP. Membranes 1 to 3 were negative controls, 4 to 6 were human serum IgG, and 7 to 9 were monoclonal IgG. (B-C) Comparison of PBS, serum IgG, monoclonal IgG, and respiratory swabs with the Sofia and Veritor assays. H1N1pdm was diluted 10-fold from stock in PBS, serum IgG, or monoclonal IgG, absorbed on a clean swab, and tested. Also, a set of samples with virus diluted in PBS was absorbed on a freshly collected NPS and tested.