Table 1.
Atherosclerosis Related Gene List (42 genes) for PCR Array.
Fig 1.
Asynchronous hemodynamics with a reversal component (OSI = 0.15) affect gene expression.
In the panels above, significant differences between SPA0 R and SPA180 R are denoted by an overbar. Other significance indicators are with respect to the static control that is indicated by a dashed line at 1. * P < 0.1, ** P < 0.05 (n = 11 for SPA = -180R and n = 6 for SPA = 0R). The (+), (-) and (±) indicate anti-atherogenic, pro-atherogenic and indeterminate, respectively.
Table 2.
Endothelial genes up-regulated by the athero-prone condition SPA = -180R relative to SPA = 0R.
Fig 2.
Comparative study of SPA0 vs SPA0R.
All of the genes that are significantly altered (p<0.1) by reversal flow under the synchronous condition (SPA0) are shown in this graph. The ratios and significance levels are given in Table 3. The (+), (-) and (±) indicate anti-atherogenic, pro-atherogenic and indeterminate, respectively.
Table 3.
Endothelial genes significantly altered by the condition SPA = 0° R relative to SPA = 0°.
Fig 3.
Comparative study of SPA 180 vs SPA 180R.
All of the genes that are significantly altered (p<0.1) by reversal flow under the asynchronous condition (SPA 180) are shown in this graph. The ratios and significance levels are given in Table 4. The (+), (-) and (±) indicate anti-atherogenic, pro-atherogenic and indeterminate, respectively.
Table 4.
Endothelial genes significantly altered by the SPA = -180° relative to SPA = -180° R.
Fig 4.
SPA and reversal flow modulate localization and protein expression of NFκB p65.
BAEC were exposed to SPA = 0°, SPA = -180°, SPA = 0°R and SPA = -180°R conditions during 7h. Both asynchronous flow and reversal flow promote nuclear translocation of NFκB p65.
Fig 5.
SPA modulates the protein expression of NFkB p65.
In the case of synchronous hemodynamics reversal flow enhanced the expression of NFkB p65 compared to non-reversing condition. In the case of asynchronous condition, reversal flow enhanced the protein expression of NFkB p65 compared to the non-reversing condition. Asynchronous hemodynamics with reversal flow modulates protein production of NFκB p65 compared to SPA = 0R. (n = 8 for each case), ** P< 0.05.