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Fig 1.

Transmission electron microscopic (TEM) images of (A) insulin and (B) lysozyme fibrils after 36 h of incubation.

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Fig 1 Expand

Fig 2.

Chemical structures of osmolytes (A) proline and (B) sorbitol.

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Fig 2 Expand

Fig 3.

Kinetics of insulin (A) and (B) and lysozyme (C) and (D) fibril extension in absence and presence of different concentration of proline and sorbitol respectively.

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Fig 3 Expand

Table 1.

Fibrillation kinetics parameter for Insulin and lysozyme.

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Table 1 Expand

Fig 4.

TEM images of insulin (A) and (B) in presence of 100 mM proline and sorbitol respectively and lysozyme (C) and (D) in presence of 500 mM proline and sorbitol respectively.

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Fig 4 Expand

Fig 5.

Kinetics of (A) insulin and (B) lysozyme fibrillation monitored by the binding of ThT with amyloid fibrils in presence of proline, sorbitol and their mixture, and TEM images of (C) insulin and (D) lysozyme in presence of mixture of proline and sorbitol.

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Fig 5 Expand

Fig 6.

Enthalpies of interaction of insulin in presence of 100 mM proline, sorbitol and their mixture at (A) native, (B) elongation and (C) saturation stages of fibrillation.

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Fig 6 Expand

Fig 7.

Comparison between sum of the enthalpies of interaction of insulin in presence of 100 mM proline and sorbitol individually and in their mixture at (A) native, (B) elongation and (C) saturation stages of fibrillation.

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Fig 7 Expand

Fig 8.

The sum of limiting standard enthalpies of interaction of insulin in 100 mM proline and sorbitol individually and in the mixture of osmolytes at different stages of fibrillation.

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Fig 8 Expand

Table 2.

The limiting standard enthalpies of interaction ().

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Table 2 Expand