Fig 1.
SNP Index and ΔSNP Index Manhattan Plots.
(a) SNP index Manhattan plot of the brown-seeded pool from the BC4 population; (b) SNP index Manhattan plot of the yellow-seeded pool from the BC4 population; (c) ΔSNP index Manhattan plot. The blue line indicates the threshold value.
Fig 2.
Combination of ΔSNP Index and Genetic Linkage Map for Brsc1 on A9 of B. rapa.
(a.) Details of the ΔSNP index on chromosome A9. Dentate lines indicate the threshold value; the two parallel black lines delineate the interval from 17.8 to 21.0 Mb on chromosome A9. (b.) Genetic linkage map of linkage markers around Brsc1 on chromosome A9. The figures on the left indicate the corresponding genetic distances (cM) between markers and Brsc1. Some markers are from previous studies (Y05 and Y06: [6]; Y14 and B089L03-3: [23]; BrA05, BrA06 and BrA09: [22]. (c.) Physical map of linkage markers for Brsc1 and BrTT1 (Bra028067). Figures on the left represent the physical locations (Mb) of linkage markers on chromosome A9. The shaded region indicates the candidate region for Brsc1. (d.) Fragment of A. thaliana chromosome 1. Orthologs of B. rapa genes are shown on the left, including TT1 (AT1G34790).
Table 1.
Sequences and Locations of SSR Markers Linked to Brsc1.
Fig 3.
Relative Expression of BrTT1 in Different Organs of BC4F2 Offspring Plants.
DAP: days after pollination. The sampling time of different organs (bud, leaf, root, flower, stem) was 7 DAP. **, extremely significant difference between BC4F2-B and BC4F2-Y plants; *, significant difference between BC4F2-B and BC4F2-Y plants.
Fig 4.
Seeds of Brown- and Yellow-Seeded Plants in BC4F2-B and BC4F2-Y Plants.
A, B, C, D, E, F: seeds of BC4F2-B plants; G, H, I, J, K, L: seeds of BC4F2-Y plants; A, G: seeds at 21 DAP; B, H: seeds at 28 DAP; C, I: seeds at 35 DAP; D, J: seeds at 42 DAP; E, K: seeds at 49 DAP; F, L: mature seeds; DAP: days after pollination.
Fig 5.
Amino Acid Sequence Alignment Between BrTT1 in B. rapa and AtTT1 in A. thaliana.
Arrows indicate single amino acid changes between BC4F2-Y (yellow) and BC4F2-B (brown) plants.