Fig 1.
CYP27B1 expression in chorionic villous and decidual tissues.
(A) RT-qPCR analysis of CYP27B1 mRNA in villous and decidual tissues obtained from normal pregnant women, PM and RM in the first trimester pregnancy. Left: chorionic villus; Right: decidua. (B) CYP27B1 protein expression in chorionic villous and decidual tissues from normal pregnant women, PM and RM. Left: chorionic villus; Right: decidua. Western blots profile of total homogenate of villous (C) and decidual tissues (D). Four representative cases of early pregnancy from normal pregnant women, PM and RM were shown. Detection of β-actin was used for protein load control.
Table 1.
Comparison of CYP27B1 mRNA in villous and decidual tissues in the control, PM, and RM group (mean±SEM).
Table 2.
Comparison of CYP27B1 protein expression in villous and decidual tissues in the control, PM, and RM group.
Fig 2.
Immunohistochemistry for CYP27B1: A1, Normal villous tissue, A2, villous tissue with RM, B1, normal decidual tissue, B2, decidual tissue with RM, C1, villous tissue without primary antibody, and C2, decidual tissue without primary antibody.
The arrows show the immunostaining cells. (Scale bar, 73 μm)
Fig 3.
CLSM for CYP27B1 and IFN-γ, IL-10: A1,B1,C1,D1,A4,B4,C4,D4,CYP27B1, A2,B2,C2,D2, IFN-γ, and A3,B3,C3,D3, overlayed CYP27B1 and IFN-γ; A5,B5,C5,D5,IL-10, and A6,B6,C6,D6, overlayed CYP27B1 and IL-10 in A1-A6, normal villous tissue, B1-B6, villous tissue with RM, C1-C6, normal decidual tissue, and D1-D6, decidual tissue with RM.
Green color indicates positive staining for CYP27B1 protein; Red color indicates IFN-γ, IL-10. The arrows show the immunostaining cells. (CLSM×400)
Fig 4.
CLSM for CYP27B1 and TNF-α, IL-2: A1,B1,C1,D1,A4,B4,C4,D4,CYP27B1, A2,B2,C2,D2, TNF-α, and A3,B3,C3,D3, overlayed CYP27B1 and TNF-α; A5,B5,C5,D5,IL-2, and A6,B6,C6,D6, overlayed CYP27B1 and IL-2 in A1-A6, normal villous tissue, B1-B6, villous tissue with RM, C1-C6, normal decidual tissue, and D1-D6 decidual tissue with RM.
Green color indicates positive staining for CYP27B1 protein; Red color indicates TNF-α, IL-2. The arrows show the immunostaining cells. (CLSM×400)