Table 1.
Characteristics of each group.
Fig 1.
Histological appearance of hematoxylin and eosin stained femoral head.
Typical images from the MC and M1 groups are shown. The diffuse presence of empty lacunae in the bone trabeculae accompanied by bone marrow cell necrosis was observed in the femoral head of M1 group. Scale bar: 100μm.
Fig 2.
Histological appearance of hematoxylin and eosin stained femoral head.
Typical images from the FC, F1, F2, F3, F4 and F5 groups are shown. Normal trabeculae and normal hematopoietic and fat cells were observed in the femoral head. Scale bar: 100μm.
Fig 3.
The percentage of empty lacunae in the femoral head and NASH steatosis score in the liver.
The percentage of empty lacunae in the M1 group was significantly higher than in the other groups (p<0.0001). The percentage of empty lacunae in the M1 group with ONFH was significantly higher than in the M1 group without ONFH (ONFH- vs ONFH+: 7.62±0.82 vs 73.29±4.34, Fig 3). Hepatic steatosis score in the M1 group was significantly higher than in the other groups (p<0.0001). † vs MC, FC, F1-5, p <0.0001, ‡ vs ONFH-, p <0.0001, Mann-Whitney test, § vs MC, FC, p <0.05.
Fig 4.
Plasma concentration of AST, ALT, TG, TC and HDL.
In the M1 group, all the parameters except TG were significantly higher compared to the MC group (P<0.0001); AST, ALT and HDL were significantly higher compared to the F1 group (P<0.0001). † vs MC, FC, F1-5, p <0.0001, ‡ vs FC, p <0.0001, § vs FC, p <0.05, ¶ vs MC, p <0.01, †† vs MC, FC or F2-5, p <0.0001, ‡‡ vs FC, p <0.01, §§ vs MC, FC, F5, p <0.0001, vs F2-4, p <0.01, ¶¶ vs MC, FC, F1, 3–5, p <0.0001, vs F2, p <0.001 ††† vs MC, p <0.0001, ‡‡‡ vs MC, p <0.05,
Fig 5.
Histological appearance of hematoxylin and eosin stained liver.
The M1 group liver shows moderate hepatocellular steatosis. Scale bar: 100μm.