Table 1.
Primers used for real-time PCR.
Fig 1.
L02 cell steatosis and POP expression after FFA treatment for 24 h.
(A-B) Oil red O staining of the control (A) and free fatty acid-treated cells (B). (C) Triglyceride concentrations in the control and FFA-treated cells. (D-E) POP mRNA expressions (D) and protein levels (E) in the control and FFA-treated cells. CON: control; FFA: free fatty acids; TG: triglyceride. * P < 0.05, ** P < 0.01, *** P < 0.001.
Fig 2.
Effects of the POP inhibitor S17092 on L02 proliferation and apoptosis with and without FFA.
(A) The proliferation of L02 cells treated with 0.026–130 μM S17092 with or without FFA for 24 h. (B) The proliferation of L02 cells treated with 0.026–130 μg/ml S17092 with or without FFA for 48 h. (C) The apoptosis rates of the L02 cells treated with S17092 with or without FFA for 24 h. (D) Intracellular POP activity in the control and FFA-treated L02 cells after incubation with S17092 at 0.026–26 μM for 2 h. (E) The intracellular AcSDKP level in the control and FFA-treated L02 cells after incubation with S17092 at 0.026–26 μM for 24 h. * P < 0.05, ** P < 0.01, *** P < 0.001.
Fig 3.
Expressions of genes involved in lipid metabolism in L02 cells with or without POP inhibition by S17902.
(A-F) Oil red O staining of control cells (A), FFA-treated cells (B), cells treated with FFA+ S17902 (0.026 μM), (C) FFA+ S17902 (0.26 μM), (D) FFA+ S17902 (13 μM), (E) or FFA+ S17902 (26 μM) (F) for 24 h; (G) TG concentration in the L02 cells treated with S17092 for 24 h.(H) FASN/PPAR-γ/SREBP-1c mRNA expression levels in the controls and FFA-treated L02 cells with or without S17092 incubation for 24 h. (I-J) LC3B II protein expression levels in the controls and FFA-treated L02 cells with or without S17092 incubation for 24 h. * P < 0.05, ** P < 0.01, *** P < 0.001.