Table 1.
Abbreviation of behavioural variables scored during testing.
Table 2.
Description of factors and factor loadings obtained by PCA using Oblimin rotation on behavioural variables assessed by OF and EPM test.
Table 3.
Description of factors and factor loadings obtained by PCA using Oblimin rotation on behavioural variables assessed by SI test.
Table 4.
The serum paroxetine levels assessed by UHPLC-MS/MS.
Fig 1.
Effect of age, genotype and treatment on performance in OF (open field) and EPM (elevated plus maze) tests, analysed by principal component analysis.
Factor scores for 4 types of behaviour (principal components) are shown in A-D. MANOVA was used to analyse significance in effect of age, genotype and treatment, followed by Fisher’s LSD post hoc test for group-wise comparisons: *, **, ***—p < 0.05, 0.01, 0.001 correspond to differences in factor scores between groups at 9, 12, 15 or 18 months of age; +, ++, +++—p < 0.05, 0.01, and 0.001 correspond to age-effect within groups compared to factor scores at 9 months; ¤, ¤¤, ¤¤¤—p < 0.05, 0.01, 0.001 correspond to intra-group comparisons of factor scores at 12 months. Bars indicate Means ± SEM Black bars, Wt (n = 14–15); white bars, Tg (n = 12–13); dark grey bars, Wt-paroxetine treated (n = 14–16); light grey bars, Tg-paroxetine treated (n = 6–12, and n = 4 at 12 months of age). 9, 12, 15 and 18 months of age correspond to 0, 3, 6 and 9 months of treatment, respectively.
Fig 2.
Effect of age, genotype and treatment on social interaction, tested on two consecutive days, analysed by principal component analysis.
Factor scores for 4 types of behaviour (principal components) are shown in A-D. MANOVA was used to analyse significance in effect of age, genotype and treatment, following by Fisher’s LSD post hoc test for group-wise comparisons: *, **, ***—p < 0.05, 0.01, 0.001 correspond to differences in factor scores between groups at 9, 12, 15 or 18 months of age; +, ++, +++—p < 0.05, 0.01, and 0.001 correspond to age-effect within groups compared to factor scores at 9 months; ¤, ¤¤, ¤¤¤—p < 0.05, 0.01, 0.001 correspond to intra-group comparisons of factor scores at 12 months. Bars indicate Means ± SEM Black bars, Wt (n = 15–23); white bars, Tg (n = 12–22); dark grey bars, Wt-paroxetine treated (n = 14–16); light grey bars, Tg-paroxetine treated (n = 7–12, and n = 3 at 12 months of age). 9, 12, 15 and 18 months of age correspond to 0, 3, 6 and 9 months of treatment, respectively.