Table 1.
Bacterial strains used in this study.
Table 2.
Composition and characteristics of the bacterial media used in this study.
Fig 1.
Bacterial growth kinetics of the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives in TY (A) and 79CA (B) media. Solid lines, OD600 of bacterial cultures; dashed lines, percentage (%) of live cells in bacterial populations.
Fig 2.
Electrophoretic mobility and the amount of EPS produced by the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives in TY (A) and 79CA (B) media after 24, 48, and 96 h. The intensity of bar color for individual strains increases with bacterial culture age.
Fig 3.
Distribution of electrophoretic mobility of 24-h cultures of the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives at OD600≈0.4 in TY (A) and 79CA (B) media.
Fig 4.
Distribution functions [f(pKapp)] of the apparent dissociation constants (pKapp) of surface functional groups of Rt24.2, Rt5819, Rt2472, Rt24.2(pBA1), and Rt24.2(pBR1) strains grown in TY (A) and 79CA (B) media.
Table 3.
Average apparent dissociation constants (pKapp,av) for the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives grown in TY and 79CA media.
Table 4.
Contact angle (CA) values for the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives.
Table 5.
Surface free energy (SFE) and its components determined for the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives.
Table 6.
Free energy of interaction between cell surfaces of two bacterial cells immersed in water (ΔGBWB) calculated for the wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives cultured in TY and 79CA media.
Fig 5.
The effect of pH (3–10) on the viability of Rt24.2, Rt5819, Rt2472, Rt24.2(pBA1), and Rt24.2(pBR1) strains grown in TY (A) and 79CA (B) media and on the electrophoretic mobility of bacterial cells in TY (C) and 79CA (D).
Table 7.
Correlation coefficients (R) for EM values and the percentage of live cells in bacterial cultures of R. leguminosarum bv. trifolii strains.