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Fig 1.

Eternal morphology of kinorhynchs.

A. Pycnophyes kielensis, scale bar = 500 μm; B. Echinoderes svetlanae, scale bar = 200 μm. Heads orient down.

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Table 1.

Nucleotide composition characteristics of E. svetlanae and P. kielensis mitochondrial genomes.

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Table 2.

GC% contents of E. svetlanae and P. kielensis mitochondrial genomes.

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Table 3.

Pycnophyes kielensis genome organization.

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Table 4.

Echinoderes svetlanae genome organization.

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Table 5.

GC% in the three codon positions of E. svetlanae and P. kielensis PCGs.

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Fig 2.

Predicted mitochondrial methionine tRNAs of Echinoderes svetlanae and Pycnophyes kielensis.

Compensatory changes are shown in red. Compensatory change in M1 –the first pair of the anticodon stem (U-A in E. svetlanae and C-G in P. kielensis). Compensatory changes in M2 –the first pair of the T-arm (U-A in E. svetlanae and C-G in P. kielensis) and the fifth pair of the anticodon stem (G-C in E. svetlanae and A-U in P. kielensis).

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Fig 3.

Bayesian tree based on the alignment of tRNA genes from E. svetlanae and P. kielensis.

Numbers at the branches indicate Bayesian posterior probabilities. Methionine tRNA genes are marked orange. tRNA specificity is coded by one letter.

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Table 6.

The codon usage in E. svetlanae and P. kielensis.

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Table 6 Expand

Fig 4.

Bayesian tree based on the concatenated dataset of 13 protein-coding genes from mitochondrial genomes after removing constant positions and fast-evolving sites from the alignment.

Numbers at the branches indicate Bayesian posterior probabilities as percent values.

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Table 7.

Start and stop codon occurrence in PCG of E. svetlanae and P. kielensis.

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Fig 5.

Protein-coding and rRNA gene orders in the mitochondrial genomes of E. svetlanae and P. kielensis.

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Fig 6.

Kinorhynch gene orders and conservative blocks of mitochondrial genes from Bilateria.

Genes and blocks are colored and named following [14].

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Fig 7.

Gene orders of E. svetlanae, Priapulida and Panarthropoda [15] with Deuterostomia [17] as an outgroup.

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Fig 8.

Putative model of gene order evolution in Protostomia reconstructed by TreeREx.

Genes are colored following [14].

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