Table 1.
Effects of SDS and NaOH on recovery of human hair shaft proteins.
Fig 1.
Temporal progress of incubation time for extracting proteins from the human hair shaft.
(a) Graph shows the percent recovery of protein obtained when hair was incubated for 5, 10, 20, 30 and 40 minutes at 90°C. Minute 0 acts as the control of the experiment, in which the incubation mixture was left at room temperature for 60 minutes. Each value represents the average percent recovery of ten individuals. (b) Representative SDS-PAGE gel showing proteins extracted using the present method when hair of an individual was incubated from 5–40 minutes in alkaline lysis buffer at 90°C, alongside with the control. Lane 1 represents control. Lanes 2 to 6 show the bands produced from 5, 10, 20, 30 and 40 minutes, respectively. (c) Raw volume of the keratin bands (Lanes 1–6) obtained from the representative figure of gel from SDS-PAGE.
Table 2.
Comparison of protein recovered from the second stage of extraction by magnetic stirring and incubation method.
Fig 2.
Outline of protein extraction procedures.
The Shindai method [5], the alkaline lysis method developed in the present study and the method of Lee et al. [9] are illustrated.
Table 3.
Recovery of hair shaft proteins using different methods of extraction.
Fig 3.
Venn diagram comparing the proteins identified from both stages of protein extraction.
Fig 4.
Pie chart showing the distribution of keratins, non-keratins and keratin-associated proteins (KAPs).
Table 4.
Identification of hair shaft proteins.