Table 1.
Summary of de novo assembly results of the transcriptome in hepatopancreas of Litopenaeus vannamei.
Table 2.
Summary of the annotations of the unigenes under ammonia stress in Litopenaeus vannamei.
Fig 1.
(a) GO categorization of non-redundant unigenes from the de novo assembly of LV_E and LV_C reads, and each annotated unigene was assigned at least one GO term. (b) KOG annotation of putative proteins. (c) KEGG classification of non-redundant unigenes.
Fig 2.
The hierarchical clustering for the differentially expressed genes between LV_E and LV_C.
The red color shows the high expression, and the blue color represents the down expression. The color from red to blue represents the log10 (FPKM+1) from large to small.
Table 3.
List of the candidate genes have homologies with known proteins in Litopenaeus vannamei under ammonia stress.
Table 4.
List of the genes have homologies to known proteins of aquatic species in significant changed GO terms under ammonia stress in Litopenaeus vannamei.
Fig 3.
The hierarchical clustering for the key differentially expressed genes between LV_E and LV_C from functional enrichment analysis of GO and KEGG pathways.
The red color shows the high expression, and the blue color represents the down expression. The color from red to blue represents the log10 (FPKM+1) from large to small.
Fig 4.
The six significant changed KEGG pathways and two nitrogen metabolism pathways: a, Linoleic acid metabolism (ko00591); b, Inflammatory mediator regulation of TRP channels (ko04750); c, Arachidonic acid metabolism (ko00590); d, Serotonergic synapse (ko04726); e, Ovarian steroidogenesis (ko04913); f, Complement and coagulation cascades (ko04610); g, nitrogen metabolism pathway (ko00910); h, alanine, aspartate and glutamate metabolism pathway (ko00250). The green frames represents the genes were down-regulated, and the red frames represents the genes were up-regulated.
Table 5.
List of the genes in significantly changed KEGG pathways under acute ammonia stress in Litopenaeus vannamei.
Fig 5.
Validation results of RNA-seq profiles by real time PCR.