Fig 1.
a. Algorithm used for identification of slowly growing nontuberculous mycobacteria in our laboratory. b. Proposed new algorithm incorporating SCGE for identification of slowly growing nontuberculous mycobacteria. Species-specific PCR; “TB/MAC” PCR, targeting M. tuberculosis [IS6110] and MAC [ITS]; “KGSA 1” PCR, targeting M. gordonae [ITS] and M. kansasii [16S]; “KGSA2” PCR, targeting M. marinum/M.ulcerans [ITS], M. asiaticum [ITS] and M. szulgai [ITS].
Table 1.
Sequencer capillary gel electrophoresis derived internal transcribed spacer-amplified fragment lengths (or peaks) of American Type Culture Collection reference strains of slowly growing nontuberculous mycobacteria.
Table 2.
Sequencer based Capillary gel electrophoresis derived internal transcribed spacer-amplified fragment lengths (or peaks) of clinical, environmental and quality assurance SG-NTM species.
Fig 2.
a. SCGE peaks of three representative ATCC strains. (i) M. asiaticum ATCC 25276 369 bp. (ii) M. avium ATCC 25291 368 bp. (iii) M. intracellulare ATCC 13950, 371 bp. b. Five SCGE fragment length types of M. avium, M. gordonae and M. intracellulare. All isolates were obtained from clinical specimen. A) M. avium SCGE type I 367.44 bp B) M. avium SCGE type II 367.75 bp C) M. avium SCGE type III 368.44 bp D) M. avium SCGE type IV 368.49 bp E) M. avium SCGE type V 369.2 bp F) M. intracellulare SCGE type I 368.44 bp G) M. intracellulare SCGE type II 370.96 bp H) M. intracellulare SCGE type III 371.17 bp I) M. intracellulare SCGE type IV 371.60 bp J) M. intracellulare SCGE type V 372.15 bp K) M. gordonae SCGE type I 360.86 bp L) M. gordonae SCGE type II 361.20 bp M) M. gordonae SCGE type III 361.50 bp N) M. gordonae SCGE type IV 362.0 bp O) M. gordonae SCGE type V 363.29 bp
Table 3.
Slowly growing mycobacterium species which demonstrated overlap of SCGE fragment lengths and the methods required to resolve species identification.
Fig 3.
SCGE fragment lengths (or peaks) of 30 different Mycoabacterium species studied to date.