Table 1.
Number of samples after each quality control step by species and method of DNA extraction.
Fig 1.
(A) Illustrative DNA Integrity gels with gel scores. Example integrity gels for (B) Holstein Friesian cattle and (C) Soay sheep. Individual samples (represented by numbers in image) that were extracted with different DNA extraction protocols. (PG: Gentra Puregene kit, SC: DNeasy spin columns, SP: DNeasy 96 well plate; GS: calibrator DNA (“golden sample”).
Fig 2.
Correlations between RTL measurements from different DNA extraction methods (PG: Gentra Puregene kit; SC: DNeasy spin columns; SP: DNeasy 96 well plate): Cattle, method-specific calibrator (A); Cattle, Puregene calibrator (B); Cattle, no calibrator (C); Sheep, method-specific calibrator (D); Sheep, no calibrator (E). Regression lines and their 95% confidence interval are shown in blue and grey, respectively, with red lines reflecting a hypothetically perfect correspondence (slope of one, intercept of zero).
Table 2.
Variance component and parameter estimates.
Fig 3.
RTL or Cq values by DNA extraction method and qPCR plate for cattle (A-E) and sheep (F-I). RTL calculated with method specific (MS) calibrator (A + F), Puregene (PG) calibrator (B), no calibrator (C+G). Cq values for telomere reaction (D+H) and control gene B2M (E+I). Colours represent DNA extraction methods. White: Gentra Puregene, blue: DNeasy spin columns, orange: DNeasy 96 well plate.