Fig 1.
Representative scatter plots of normalized miRNA expression showing the correlation between FFPE and frozen paired tumor samples.
Fig 2.
Correlation matrix heatmap showing the Euclidean distance between non-tumor bearing liver and ICC samples.
Darker color indicates stronger correlation.
Fig 3.
Heat map of the differentially expressed miRNAs (adjusted p value <0.001) between ICC and non-tumor bearing liver FFPE samples.
Fig 4.
Selected miRNAs based on the highest degree of up regulation (fold change, blue columns) and absolute expression counts (red columns).
Fold change was calculated between ICC and non tumor-bearing liver. Error bars represent standard error of the mean.
Fig 5.
RT-qPCR validation of differentially expressed miRNAs in tissue (A) and plasma (B).
(A) differential expression of miR-21, miR-34c, miR-200b and miR-221 on tissues from 10 independent patients with ICC and 10 normal liver samples. Horizontal bars represent mean value. (B) Expression levels of circulating miR-21 and miR-221 in an independent cohort of patients with ICC vs healthy controls.
Fig 6.
Receiver operating characteristic (ROC) curve for circulating miR-21 for the diagnosis intrahepatic cholangiocarcinoma.
ROC was performed for plasma miR-21 from patients with ICC vs healthy controls. Area under the curve (AUC) for miR-21 is 0.94.
Table 1.
Normalized expression of selected miRNAs in plasma.
Table 2.
Clinicopathological features of ICC patients.