Table 1.
Precision (R.S.D in %), Accuracy (%), Limit of Detection (LOD), the Lower Limit of Quantification (LLOQ), Linear Regression (LR) and Correlation Coefficient (r), for the Analyzed Catechins and Theaflavins1.
Fig 1.
Detection of Catechins and Theaflavins in Plasma Spiked with Individual Polyphenols.
Representative MRM chromatograms of epigallocatechin (EGC), epicatechin (EC), epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), theaflavin (TF), theaflavin-3-monogallate (TF3G), theaflavin-3,3'-digallate (TF33'diG), theaflavin-3'-monogallate (TF3'G), in blank plasma (control) spiked with the polyphenol standards to the final concentration of their LLOQ, showing the retention time (labelled below the analyte name) and response (labelled below the retention time) of the analytes (analyte names highlighted against their corresponding chromatograms). The right-hand top labels on the chromatograms indicate their respective MRM transitions and peak heights. Data are representative of three independent experiments done under similar conditions.
Table 2.
Bioactive Polyphenol Content of Assam and Darjeeling Black Tea infusion1,2.
Table 3.
Levels of Tea Polyphenols Detected in Guinea Pig Plasma after Oral Administration of ABT and DBT1,2
Table 4.
Levels of Tea Polyphenols Detected in Guinea Pig Lung and Kidney after Oral Administration of ABT or DBT1.
Fig 2.
Detection and Identifiction of Catechin Metabolites in the Urine of Tea-Consuming Guinea Pigs.
Representative chromatograms for the identification of catechin metabolites in the urine of tea consuming guinea pigs analysed by precursor ion scan showing epigallocatechin glucuronide (EGC-glucuronide), epicatechin glucuronide (EC-glucuronide), o-methyl epigallocatechin glucuronide (o-methyl EGC glucuronide), epigallocatechin sulphate (EGC-sulphate), epicatechin sulphate (EC-sulphate), o-methyl epigallocatechin sulphate (o-methyl EGC-sulphate), o-methyl epicatechin sulphate (o-methyl EC-sulphate) as the primarily identifiable metabolites. The chromatograms also depict the retention times (RT) (highlighted corresponding to the monitoring ions) and the intensity count (highlighted at the right hand side of the chromatograms) of the identified metabolites. Data are representative of three independent experiments done under similar conditions.