Table 1.
Summary statistics of the annotation process of the P. xanthii epiphytic transcriptome.
Fig 1.
Pie chart representing the functional annotation of predicted proteins from the epiphytic transcriptome of P. xanthii.
The diagram displays the relative abundance of C. albicans GOSlim categories among the 6,645 annotated predicted proteins. All data were submitted to the NCBI database under the accession GEUO00000000.
Fig 2.
The most expressed GO categories in the epiphytic transcriptome of P. xanthii.
Representation of the number of average reads within the most expressed GO categories in the P. xanthii epiphytic cDNA library. For each category, the number of genes within the group of Top50 most expressed transcripts is shown on the right side of the bars. See S2 Table for details on Top50 most expressed genes.
Fig 3.
Pie chart representing the functional annotation of the P. xanthii secretome deduced from the predicted proteins from the epiphytic transcriptome.
The diagram displays the relative abundance of GO categories among the total of 137 putatively secreted proteins identified; 84 secreted proteins were grouped by biological functions, and 53 had no GO terms and, consequently, were catalogued as candidate secreted effector proteins (CSEPs). The number of proteins within each functional category is presented in brackets. See Table 2 and S4 Table for details.
Table 2.
Annotation of the epiphytic secretome of P. xanthii.
Only categories “Pathogenesis” and “Host interaction” are presented. Rest of categories is shown in S4 Table.
Table 3.
List of CSEPs identified in the P. xanthii epiphytic secretome and orthologues present in the genome of B. graminis f. sp. hordei.
Fig 4.
Phylogenetic analysis of P. xanthii CSEPs.
The unrooted phylogenetic tree is based on NJ analysis. The confidence of groupings was estimated by using 1,000 bootstrap replicates, and the percentage of replicate trees supporting each branch is shown next to the branching point. The analysis showed the existence of potential families within the P. xanthii CSEPs.
Fig 5.
N-terminal amino acid Y/F/WxC motif and natural selection in P. xanthii CSEPs.
The N-terminal amino acid motif Y/F/WxC was screened manually within the pool of CSEPs, and only the motif WxC was observed following the secretion signal. Alignments were performed with ClustalW2, and the sequence logo was represented using the Seq2Logo online server (www.cbs.dtu.dk/biotools/Seq2Logo/).
Fig 6.
Expression of selected P. xanthii CSEP genes in a time-course experiment.
Total RNA was isolated from zucchini cotyledons infected with P. xanthii at different time points, and the relative expression of five putative effector-genes (CSEP01, CSEP02, CSEP05, CSEP021 and a homologue to Scp160) was analysed by quantitative RT-PCR. Transcript abundance was normalised to the endogenous control β-tubulin gene PxTUB2 (KC333362). Relative expression of each gene was calibrated to the sample 8 h post inoculation. Points represent the mean ± standard deviation of three technical replicates from two cDNA samples obtained from two different zucchini cotyledons.