Fig 1.
Thirty (30) participants were enrolled, 23 completed the study per protocol.
Fig 2.
Summary of adverse events after each vaccination.
Clinical solicited (A-C) and Laboratory (D-F) adverse events after each vaccination with each dose group are reported in individual graphs: Group 1a (A and D), 1b (B and E) and Group 2 (C and F). All adverse events were mild except when indicated as moderate.
Fig 3.
A, Groups 1a and 1b participants, receiving conjugated proteins comprising of 8 μg and 16 μg Pfs25H, respectively. B, Group 2 participants, receiving 47 μg Pfs25H. Arrows indicate the day of vaccination. Closed circles represent antibody level and individual participants, and black bars indicate geometric mean of antibody levels and t-distribution 95% confidence interval (CI).
Fig 4.
Immunofluorescence assays with immune sera.
A. Surface labeling of zygotes with sera from one volunteer (#20) collected on days 0, 314 and 356, with Pfs25 specific mouse mAbs 1G2 and 4B7. B. Recognition of parasite protein in fixed ookinetes with sera from one volunteer (#20) collected on days 0, 314 and 356, with Pfs25 specific mouse mAb 4B7. Magnification 1000X.
Fig 5.
Transmisison reducing activity of immune sera.
A. Transmission reducing activity of sera collected 2 weeks after each initial vaccination and 8 weeks after the booster vaccination. Each data point represents the average of two results in the SMFA, and lines indicate estimate EC50 and point-wise confidence interval by GEE model. B. Standard deviation (SD) of individual TRA from replica assays. Thick blue line indicates the threshold representing significant biological activity (50% TRA), the black line is a standard loess smooth curve. The green, red, blue, and black dots correspond to the data points on D70, D134, D314 (2 weeks after the second, third, and fourth vaccination respectively), and D356 (8 weeks after the fourth vaccination), as in Fig 4A.
Fig 6.
Paired comparison of Avidity Index in Sera after Subsequent Vaccinations.