Table 1.
List of primers used in the study
Fig 1.
cDNA sequence of Plutella xylostella Adenosine Receptor.
The line box area indicates the open reading frame (ORF), which encodes protein of 440 amino acids. The start codon (ATG) and the stop codon (TGA) are highlighted in black. The sequence was deposited in the GenBank (Assession No.KR258794).
Table 2.
The similarity of protein sequences between PxAdoR and other insect adenosine receptors.
Fig 2.
Alignment of the human A2b adenosine receptor and N-terminal part of AdoR amino acids sequences of other insects and Plutella xylostella.
Different color shows different homology levels. Black means 100% homology level, pink shows 75% homology level, blue stands for 50% homology level, and yellow marks 33% homology level. The PxAdoR and human adenosine receptors share the same key residues marked by red solid border and the mismatched key residues between PxAdoR and human adenosine receptors are marked by black solid border. “#” sign indicates the special residues of PxAdoR compared with human adenosine receptors.
Fig 3.
(A) Effect of PxAdoR silencing by 500 ng/mL dsRNA feeding. (B) Effect of HEA feeding after silencing PxAdoR. Analyzed sample were cDNAs that were reverse-transcribed from pooled RNA samples of 10 larvae. Bars represent the means ± standard deviations of technical replicates. *P < 0.05; **P < 0.001 (A) compared with the dsgfp group. (B) compared with the control.
Table 3.
The effect on relative PxAdoR mRNA level by qRT-PCR.
Fig 4.
Impact of RNAi and Receptor Inhibitor on the insecticidal effect of HEA.
Larvae were fed with dsRNA or receptor inhibitor (SCH58261) with or without HEA for a period of 24 h to 60 h. *P < 0.05; **P < 0.001 compared with the HEA group. (A) Effect of PxAdoR silencing and SCH58261 on the mortality induced by HEA. Corrected mortality (%) = (mortality of treatment−mortality of control)/(1−mortality of control)×100. (B) Effect of PxAdoR silencing on the rate of larvae growth. Rate of growth inhibition (%) = [(weight of control−weight of treatment)/ weight of control]×100.