Fig 1.
Conversion of 12-oxophytodienoic acid (OPDA) in leaves of Arabidopsis thaliana ecotype WS (wild type, A) and opr3 (B).
A, Leaves of 5-week-old WS plants grown under short-day conditions were floated on water (control, black bars) or on 50 μM OPDA for the time periods indicated. Levels of OPDA, jasmonic acid (JA) and JA-Ile are presented. B, Leaves of 5-week-old opr3 plants grown under short-day conditions were floated on solutions containing different concentrations of OPDA for 30 min. Levels of OPDA (red bars), JA (blue bars) and JA-Ile (green bars) are presented. Contents of OPDA, JA and JA-Ile were determined by LC-MS according to [30]. Each value is represented by the mean of three independent biological replicates ± SD. Treatments and controls were pairwise compared by the Student’s t-test, *p≤0.05, **p≤0.01, ***p≤0.001.
Fig 2.
Levels of OPDA (A), OPDA-Ile (B), JA (C) and JA-Ile (D) in seedlings of Arabidopsis thaliana WS, opr3, Col-0 and jar1 after treatment with OPDA or OPDA-Ile.
10-days-old seedlings grown in liquid culture were treated with 30 μM OPDA or 30 μM OPDA-Ile for 30 min (white bars). Two independent controls (black bars) were performed by treatment with bi-distilled H2O containing 0.87% [v/v] and 0.56% [v/v] acetonitrile, respectively. Compounds were quantified according to [24]. Each value is represented by the mean of five independent biological replicates ± SD. Treatments and controls were pairwise compared by the Student’s t-test, *p≤0.05, **p≤0.01, ***p≤0.001.
Fig 3.
Relative transcript levels of GRX480 (A) and ZAT10 (B) in seedlings of Arabidopsis thaliana WS, opr3, Col-0 and jar1 after treatment with OPDA or OPDA-Ile.
10-days-old seedlings grown in liquid culture were treated with 30 μM OPDA or 30 μM OPDA-Ile for 30 min (white bars). Two independent controls (black bars) were performed by treatment with bi-distilled H2O containing 0.87% [v/v] and 0.56% [v/v] acetonitrile, respectively. Relative transcript levels were quantified by qRT-PCR using AtPP2A as reference. Each value is represented by the mean of three independent biological replicates ± SD. Treatments and controls were pairwise compared by the Student’s t-test, *p≤0.05, **p≤0.01, ***p≤0.001.