Fig 1.
Representative differential interference contrast microscope images of erythrocytes examined in this study.
Normal erythrocytes, erythrocytes from an HbSS patient with both reversibly sickled cells (RSC) and irreversibly sickled cells (ISC), and HbSC erythrocytes are shown in separate panels.
Fig 2.
Representative trajectories of DIDS-biotin labeled band 3 on intact normal and sickle human erythrocytes.
After labeling with DIDS-biotin conjugate, the diffusion of the labeled band 3 was imaged for 100 consecutive frames at 120 frames/sec on intact fixed normal red blood cells, unfixed normal erythrocytes, HbSC erythrocytes, reversibly sickled cells (RSC) or irreversibly sickled cells (ISC). The different colors track band 3 diffusion as a function of time, beginning with blue and progressing through the colors of the rainbow to red. Because all of these erythrocytes have multiple subpopulations of band 3, the trajectory of only the most abundant population is displayed (% of total band 3 is indicated in parentheses).
Fig 3.
Distributions of the logarithms of the microscopic (Dμ) and macroscopic (DM) diffusion coefficients of band 3 in healthy and sickle erythrocytes.
Diffusion coefficients were determined by analysis of individual trajectories of labeled band 3 molecules in intact fixed normal cells, unfixed normal cells, reversibly sickled cells (RSC), irreversibly sickled cells (ISC), and HbSC erythrocytes.
Table 1.
Microscopic and macroscopic diffusion coefficient data for various healthy and sickle cell erythrocytes populations.
Fig 4.
Distribution of the compartment sizes in intact healthy and sickle erythrocytes.
Compartment sizes were determined by analysis of individual trajectories of labeled band 3 molecules in intact unfixed normal cells, reversibly sickled cells (RSC), irreversibly sickled cells (ISC), and HbSC cells.