Fig 1.
Kinetics of Listeria-specific CD8 T cells in WT and E-FABP-deficient mice.
WT and E-FABP-/- mice were infected with 5x106 Lm-OVA. OVA-specific CD8 T cells were detected by MHC class I tetramer (Kb-OVA257-264) staining (A-B) or peptide (OVA257-264) stimulated intracellular cytokine staining for IFN-γ (C-D). (A and C) Representative contour plots showing the frequency of OVA-specific CD8 T cells, amongst all CD8+ cells, at the indicated days following infection with Lm-OVA. (B and D) Total number of OVA-specific CD8 T cells in the spleen. Data (mean±s.d.) are from 3-mice/time point/group except day 63 E-FABP-/- (n = 2). Data are representative of two independent experiments and were analyzed by the Mann-Whitney test.
Fig 2.
Phenotypic and functional characterization of Listeria-specific CD8 T cells.
(A, C, E, G) Representative histograms and contour plots from day 7 or day 63 showing the frequency of OVA-specific CD8 T cells that are positive for the indicated cell surface marker or cytokine. Open histogram is isotype control; gray histogram is CD43 (clone 1B11). (B, D, F, H) Total percentage of OVA-specific CD8 T cells positive for the indicated cell surface marker or cytokine on the indicated days. Data (mean±s.d.) are from 3-mice/time point/group except day 63 WT (n = 2). Data are representative of two independent experiments and were analyzed by the Mann-Whitney test.
Fig 3.
Kinetics of Listeria-specific CD4 T cells in WT and E-FABP-deficient mice.
WT and E-FABP-/- mice were infected with 5x106 Lm-OVA. (A) Representative contour plots showing the frequency of LLO-specific CD4 T cells as detected by peptide (LLO190-201) stimulated intracellular cytokine staining for IFN-γ at the indicated days following infection with Lm-OVA. (B) Total number of LLO-specific CD4 T cells in the spleen. Data (mean±s.d.) are from 3-mice/time point/group except day 63 WT (n = 2). Data are representative of two independent experiments and were analyzed by the Mann-Whitney test.
Fig 4.
Phenotypic and functional characterization of Listeria-specific CD4 T cells.
(A, C, E) Representative histograms and contour plots from day 7 or day 63 showing the frequency of LLO-specific CD4 T cells that are positive for the indicated cell surface marker or cytokine. (A) Open histogram is isotype control; gray histogram is CD43 (clone 1B11). (C) Open histogram is isotype control; gray histogram is CD27. (B, D, F) Total percentage of LLO-specific CD4 T cells positive for the indicated cell surface marker or cytokine on the indicated days. Data (mean±s.d.) are from 3-mice/time point/group except day 63 WT (n = 2). Data are representative of two independent experiments and were analyzed by the Mann-Whitney test.